INVESTIGADORES
BUTLER Matias
congresos y reuniones científicas
Título:
Molecular radical cations and fragmentation studies of nitrosylated peptides containing cysteine and tryptophan
Autor/es:
BUTLER, M.; SIU, K.W.M; HOPKINSON, A.C.
Lugar:
Orillia
Reunión:
Conferencia; 31 Annual Trent Conference on Mass Spectrometry; 2014
Resumen:
Molecular radical cations and fragmentation
studies of nitrosylated peptides containing Cysteine and Tryptophan
Cysteine and tryptophan side-chains can be
nitrosylated leading to the formation of chemically modified peptides. Upon
Collision-Induced Dissociation (CID) of the protonated peptides generated by Electrospray
Ionization (ESI), the labile NO group is easily lost thus generating radical
cations. Copper complexation strategies combined with ESI are used as an
alternative method for obtaining structurally different radical cations from
the unmodified peptides. The fragmentation behavior in the positive ion mode of
the radical cations prepared by the two different routes from model peptides is
compared. The behavior of ?di-radical? cations generated from peptides
containing two modified residues of cysteine and/or tryptophan is analyzed as
well to explore the possibility of creating diradicals and ascertain their resemblance
to open- or closed-shell species.