Improving the Properties of Chitosan as Support for the Covalent Immobilization of L-arabinose isomerase

MANZO, RICARDO M.; FENOGLIO, CECILIA L.; RUBIOLO, AMELIA C.; MAMMARELLA, ENRIQUE J.

Foz do Iguacú

Simposio; XIX Simposio Nacional de Bioprocesos y en el X Simposio de hidrólisis enzimática de biomasas; 2013

Asociación Brasilera de Ingeniería Química (ABEQ), CAPES, CNPq, FAPESP

Changing gel structure led to a significant improvement in the covalent multipoint attachment of L-arabinose isomerase (L-AI) on chitosan. The use of D-lactose, D-fructose, polyvinyl alcohol, sodium tripolyphosphate, aluminium polychloride, dimethylformamide or 2,4,6-trinitrobenzene sulfonic acid and the activation with glutaraldehyde allowed the modification of the gel structure. Immobilization yields and recovered activities were evaluated and compared with commercial supports of glyoxyl activated agarose gel. Enzyme immobilization in N-alkyl chitosan derivative produced by fructose addition has conducted to the best chitosan derivative. This support allowed an enzyme load up to 9.3 mg L-AI per ggel with a specific activity of 88.4 U ggel-1, while the best commercial glyoxyl activated agarose support allowed an enzyme load up to 28.9 mg L-AI per ggel with a specific activity of 153.8 U ggel-1.