Argentinean propolis associated to Larrea nitida Cav. exudate

AGÜERO, MARÍA BELÉN; SVETAZ, LAURA; SÁNCHEZ, MARIANELA; LIMA, BEATRIZ; ZACCHINO, SUSANA; PALERMO, JORGE; WUNDERLIN, DANIEL; FERESIN, GABRIELA; TAPIA, ALEJANDRO

Mendoza

Congreso; XXVIII Annual Scientific Meeting (In memoriam Dr. Juan Carlos Fasciolo); 2010

SOCIEDAD DE BIOLOGíA DE CUYO

We report the in vitro antifungal activity of propolis extracts from the province of San Juan (Argentina) as well as the identification of their main antifungal compounds and botanical origin. The antifungal activity was determined by the microdilution technique, using reference microorganisms and clinical isolates. All dermatophytes and yeasts tested were strongly inhibited by different propolis extracts and Larrea nitida exudate (MICs between 31.25 and 125μg/mL). The most susceptible yeast was Cryptococcus neoformans (31.25μg/mL).The main antifungal compounds were methylnordihydroguaiaretic acid (MNDGA) 1, nordihydroguaiaretic acid (NDGA) 2 and the MNDGA derivative 3. Both ligans 1, 2 displayed strong activity against clinical isolates of Candidas spp, Cryptococcus spp, Trichophyton rubrum and T. mentagrophytes (MICs and MFCs between 31.25 and 62.25 μg /mL). Additionally, two epoxylignan were isolated from propolis samples and Larrea nitida exudates, showing moderate antifungal activity. This is the first report matching the chemical profile of L. nitida exudates with their corresponding propolis by HPLCMS, giving strong evidences on the botanical origin of studied propolis from Argentina.