INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Isolation and biochemical characterization of rhizosphere bacteria as potential plant growth promoters
Autor/es:
LAMI MJ1, COSTA GUTIERREZ SB1, CARAM DI SANTO MC1, POMARES F1, VINCENT PA1, ESPINOSA URGEL M2 Y DE CRISTÓBAL RE1.
Lugar:
La Falda- Cordoba
Reunión:
Congreso; CONGRESO INTERNACIONAL PGPR; 2014
Resumen:
Introduction
Great
part of problems that humanity faces are due to the degradation and loss of
arable land and organic carbon content, the loss of structural stability, the
decrease in soil biological activity, and the increase risk of erosion and
salinization. It is well known that a considerable number of
bacterial species, mostly those associated with the plant rhizosphere, are able
to exert a beneficial effect upon plant growth. Therefore, their use as
biofertilizers or control agents for agriculture improvement has been a focus
of numerous researchers, possible
solution to these problems is the use of rhizosphere bacteria that stimulate
plant growth as bioinoculants.
Objective
Isolate and
identify potential plant growth promoting rhizobacteria stems of saline soils
and biochemically characterization as them.
Materials and Methods
Root samples were from Argentine Salinas,
Salinas Grandes of Santiago del Estero and Antinaco Valley of La Rioja. The
isolation was based on differences in the morphology of their colonies. Isolates
were identified by sequencing the 16S gene and their characterization was
carried out by determining the ability of solubilizing the inorganic phosphate
by growing on solid medium with tricalcium phosphate (NBRIP supplemented), total
indol production was determined by the colorimetric reaction with the reagent
Salkowsky, and resistance to 1.2 M NaCl was analyzed in solid LB medium. Pseudomonas putida was used as positive
control.
Results
A total of 25 strains were isolated, 20 of them
were able to solubilized phosphate with an efficiency between 3.33 to 4.02%,
compared to the control that was 2.92%. There were 14 strains producing
siderophores, one of them have a greater efficiency of 350% compared to 217% of
control. The other aislates had similar values to the control. The total
production of indoles were between 0.17 to 24 ug/mL. Finally, 9 strains grown optimally
with NaCl (mass and isolated colonies), 8 only grown on ground and the other were
not able.
Conclusion
Potentially promoting plant growth rhizosphere
strains were isolated. Strains 2, 17S, 22S, 33S, 34S from the Salinas Grandes
and 41S from Antinaco Valley, are those with increased activity in all
biochemical tests and resistance to NaCl.
Áreas temáticas propuestas: Ecología de la rizósfera (3) y Aplicaciones
agronómicas (5).