INVESTIGADORES
FANARA Juan Jose
congresos y reuniones científicas
Título:
Genetic basis of adaptive developmental traits in Drosophila melanogaster: phenotypic variation and plasticity in natural-derived populations
Autor/es:
PETINO ZAPPALA, A; ORTIZ, V; SATORRE, I.; MENSCH, J; CARREIRA, VP; FANARA JJ
Lugar:
Raleigh
Reunión:
Congreso; Evolution meeting; 2014
Institución organizadora:
Society for the Study of Evolution
Resumen:
Genetic
basis of adaptive developmental traits in Drosophila melanogaster: phenotypic
variation and plasticity in natural-derived populations
Alejandra Petino Zappala mapz@ege.fcen.uba.ar
Victoria Ortiz, Universidad de Buenos Aires,
vicrotas@gmail.com
Ignacio Satorre, Universidad de Buenos Aires, Argentina,
ignaciosatorre@hotmail.com
Julián Mensch, Universidad de Buenos Aires, Argentina,
jmensch@ege.fcen.uba.ar
Valeria Carreira, Universidad de Buenos Aires,
vpcarreira@ege.fcen.uba.ar (Presenter)
Juan José Fanara, Universidad de Buenos Aires, Argentina,
jjfanara@ege.fcen.uba.ar
The study
of the genetic basis of phenotypic variation in natural populations is critical
to understand the mechanisms and processes involved in adaptation. Here we show
results addressed to analyze the genetic variability, phenotypic plasticity and
environmental sensitivity for developmental adaptive traits in D. melanogaster.
We utilized isogenic lines derived from two nearby natural populations of
Argentina located at different altitude as well as isogenic lines of the
Drosophila Reference Genome Panel derived from Raleigh (NC) that was considered
also as a lowland population. Larval and pupal Developmental Times (DTs) were
estimated in individuals reared at different temperature (25ºC and 17ºC) to
investigate developmental plasticity whereas the robustness was calculated by
means of their environmental variability. Larval and pupal DT exhibited a
particular variation pattern for each population that differs between these
developmental stages suggesting a decoupling between larval and pupal DT. Since
these results are consistent with previous observations, where lines derived
from different populations and mutagenized lines were analyzed, we considered
larval and pupal DT as different traits. These two traits presented significant
genetic variation among populations as well as differences in their robustness
although the levels of plasticity were similar among them. We observed that the
populations differ in their DT and that differences are temperature specific.
In fact, lines from lowland population of Argentina showed higher DTs when
larvae were reared at 17º whereas at 25ºC the highest DT corresponds to lines
derived from the highland population. Our results indicate that these developmental
traits showed specific temperature-populations dynamics. We will perform GWAS
in these lines to compare the genetic basis of phenotypic adaptive
developmental traits variation among all three populations and associate these
variations with ecological diversity.