INFLUENCE OF Lactobacillus casei CRL 1505 ON HEMOSTATIC AND HISTOLOGICAL ALTERATIONS IN A PNEUMONIA MODEL IN MALNOURISHED MICE

ZELAYA H.; HARO C.; LAIÑO J.; ALVAREZ S.; G. AGÜERO

Tafí del Valle. Tucumán, Argentina

Jornada; XXV Jornadas Científicas de la Asociación de Biología de Tucumán; 2008

Asociación de Biología de Tucumán

Aim: to evaluate if the oral (LcOral) or nasal (LcNasal) addition of Lactobacillus casei CRL 1505 contributes to modulating the hemostatic and histological alterations in a pneumonia model in malnourished mice. Malnourished mice received 7d balanced conventional diet (BCD) or BCD with LcOralor LcNasal, during the last 5d. These animals and malnourished controls (MNC) were infected with Streptococcus pneumonia (105 CFU/mouse). At the 0, 1, 5 and 10d post-infection the animals that received different diets showed: increase of white cells number (nx107cells/L) in broncho-alveolar lavage (BAL) (5dpi: MNC=13±0,8; BCD=19,5±1,2; Lcnasal=60±1,3; Lcoral=40±1,2); lower albumin concentration in BAL; lower histological alterations in lung; diminution of the Fibrinogen (F) deposition in lung; partial recovery of prothrombin activity (5dpi: MNC=28,81±3,53%; BCD=53,3±5,77%; Lcnasal=80±4,95%; Lcoral=56±4,55%), lower alteration of activated partial thromboplastin time, increment in F levels and platelets count, respect to MNC. Conclusions:All diets limited the damage induced by the pathogen, but the treatment with Lactobacillus casei was more effective specially when was administered intranasally.