ACUTE PHASE PROTEINS AND ACTIVATION OF COAGULATION IN AN EXPERIMENTAL PNEUMONIA IN MALNOURISHED MICE. EFFECT OF Lactobacillus casei

ZELAYA H; LAIÑO J; HARO C; MARQUEZ A; ALVAREZ S; G AGÜERO

Tafi del Valle

Jornada; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; 2009

Asociación de Biología de Tucumán

Aim: to evaluate if addition of Lactobacillus casei CRL 431 (Lc) to a diet of renutrition modulates the behaviour of acute phase proteins (APP), which play an important role in the activation of coagulation in a pneumonia model in malnourished mice. Malnourished mice received 7d balanced conventional diet (BCD) or BCD with Lc during the last 2d (BCD+Lc). BCD, BCD+Lc, malnourished (MNC) and wellnourished controls (WNC) were infected with Streptococcus pneumoiae. Results: At 0 h post infection (hpi) malnutrition altered all the parameters and renutrition with Lc normalized it. The infection induced in MNC decrease in Total protein (TP) and Albumin (A), increased Thrombin antithrombin complexes (TATc), Fibrinogen (F) and á-Acid glicoprotein (AGP), while DBC+Lc normalized TP, A, TATc (TATc120hpi MNC=25±0,08 mg/L; WNC= 13,99± 1,20; BCD=25,50±1,53; BCD+Lc=7,6±1,53), F (F120hpi MNC= 180,12±4,96 mg/dL; WNC=304,61±2,12; BCD=260±1,18; BCD+Lc= 239,50±6,92) and showed lower concentrations of AGP. Conclusions: supplementation with Lc a diet renutrition modulatesthe behaviour of the tested APP, leading to less activation ofcoagulation. Thus, Lc contributes to modulating the balance inflammation-hemostasis in a pneumonia model in malnourished mice.