Statistical properties of the neuronal discharge along a surgical tract in the normal rat under chloral-hydrate anesthesia. (Póster)

ANDRÉS D.; BOCACCIO H.; CERQUETTI D.; MERELLO M.; STOOP R.

Dublin

Congreso; 16th International Congress of Parkinson's Disease and Movement Disorders; 2012

The Movement Disorder Society (MDS)

Objective: The objective of the present work is to describe the statistical properties of the neuronal discharge along a surgical tract in the normal rat under hydrate-chloral anesthesia. Background: The correct identification of the surgical target during stereotactic surgery is an important goal during the implantation of DBS electrodes that is often assisted by neuronal microrecording techniques. Currently there does not exist a standardized protocol to achieve that goal. Furthermore, it is commonly assumed that microrecording techniques can only be helpful in alert patients and not under the effects of anesthesia. We analyzed the statistical properties of the neuronal discharge along a surgical tract in the normal anesthetized rat to address the issue of the possible usefulness of neuronal microrecording for target identification under anesthesia. Methods: We studied neuronal recordings from normal adult Sprague-Dawley rats anesthetized with intraperitoneal hydratechloral (300 mg/kg). The recordings were obtained with Pt/Ir (80/20%) electrodes with a nominal impedance of 0.8-1.2 MegaOhms. Single units were extracted off-line and interspike intervals (ISIS) time series were constructed for the analysis. We calculated the mean, standard deviation, asymmetry, kurtosis, rank and percentiles for cortical, hippocampal, thalamic and pallidal (MGP) neurons. Results: A total of 25 recordings obtained from 12 animals were studied. The mean frequency was lower in the MGP neurons and the rank was larger compared to hippocampal and thalamic neurons. Assymetry and kurtosis were lower in the thalamic neurons. Conclusions: Different structures along the surgical tract studied had characteristic ISIS distributions. This fact might be useful for the detection of the surgical target assisted by microrecording even when conducting surgery under anesthesia. Previously Presented: The present work is the product of original research and has not been previously presented or published.