Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei

GAVEGLIO, V.L.; GIUSTO, N.M.; PASQUARÉ, S.J

Boston

Congreso; Annual Meeting of AAA,APS,ASBMB,ASIP,ASN,ASPET; 2013

FASEB

Nuclear lipid metabolism gives rise to several lipid second messengers that seem to be involved in the regulation of nuclear structure and gene expression. The purpose of the present research was to study the metabolic pathways involved in the metabolism of phosphatidic acid (PA) and its regulation in isolated nuclei from the central nervous system. This study was also conducted in isolated nuclei from aged animals, thus allowing us to analyze the effects of neurodegeneration processes on PA metabolism as a result of aging. Adult (4 mo) and aged (28 mo) rat cerebellums were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using radiolabelled substrates we demonstrated lipid phosphate phosphatase, diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), lysophosphatidate phosphohydrolase and phospholipase as well as phospholipase type A activities. We further studied their regulation by retinoic acid (RA) which was observed to decrease DAGL, MAGL and PA-PLA activities. Significant aged-related changes in the above-mentioned enzymatic activities as well as in their regulation by RA were observed. Taken together, our results demonstrate a RA-regulated PA metabolism in rat cerebellum nuclei which could be involved in neurodegeneration processes. This research was funded by: CONICET, FONCyT, SCyT-UNS.