Effect of progesterone on the nigrostriatal metabolic activity of hemiparkinsonian male rats

GAGLIO, E; CASAS, S; HERRERA, M; GARCIA, S; CABRERA, R; YUNES, R

Huerta Grande, Córdoba

Congreso; XXVIII Congreso de la Sociedad Argentina de Neurociencias; 2013

Sociedad Argentina de Neurociencias

Previously we reported that hemiparkinsonian (HP) male rats have motor dysfunctions and alterations in the striatal neuronal activity. We also demonstrated that progesterone (P4) s.c. reverts some of these effects. We hypothesize that those changes could affect the metabolic activity (MA) of left corpus striatum (lCS) and left substantia nigra (lSN). In this work we evaluated the MA in lCS and lSN of HP male rats obtained by lesion with the neurotoxic 6-OHDA. We used the method of chemical reduction of 3-(4,5-dimetyltiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The groups we used were: G1) Sham rats; G2) HP rats and G3) HP rats treated with P4 s.c. for 3 consecutive days (4 mg/kg/day). Eight weeks after lesion, the rats were killed, their lCS and lSN dissected out and used for MTT assay. The results were expressed as MA per mg of protein (media ± SEM) and analyzed by ANOVA 1. In lSC the MA was reduced in G3 regarding G1 and G2 (p