IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Regeneration of the Olfactory Epithelium. Role of Wnt and
Autor/es:
FRONTERA, J.; CERVINO A; PAZ DA
Lugar:
Huerta Grande. Córdoba
Reunión:
Congreso; XXVIII Encuentro Anual. Sociedad Argentina de Neurociencias (SAN); 2013
Institución organizadora:
Sociedad Argentina de Neurociencias
Resumen:
Cellular and Molecular
Neurobiology
Poster Number 25 /
Session 1
Regeneration of the
Olfactory Epithelium. Role of Wnt and
Sox2 in the
differentiation of the olfactory receptors neurons
Jimena L. Frontera, Ailen Cervino, Dante A. Paz
Laboratorio de Biología del Desarrollo, IFIByNE
(UBA-CONICET)
jimenafront@gmail.com
________________________________________________________
The olfactory epithelium
(OE) has been known for its capability of actively
generating olfactory
receptor neurons throughout adulthood, and rapid neuronal
regeneration after
extensive damage to the tissue, due to the presence of neural
stem cells (NSC) in the
basal layer of the OE. Wnt promotes cell proliferation and
regulates neurogenesis in
multiple tissues. In the other hand, during neurogenesis,
Sox2 antagonizes proneural
genes in order to maintain progenitors. Therefore, in
the last years a
directional Wnt-Sox2-proneural pathway has been investigated in
order to describe the
regulation of the transition from proliferation to
differentiation of NSC .In
the present study, we analyzed the Sox2 expression in the
olfactory basal cells and
the activation of Wnt signaling pathways in normal and
regenerating OE. We found
that sox2 expression is significantly increased during
extensive regeneration and
would be regulated by Wnt. Immunohistochemistry
and qRT-PCR showed that
after Wnt inhibition, sox2 expression is significantly
decreased during OE
regeneration. Analysis of Wnt/B-catenin signaling pathway
showed that B-catenin is
not trans-located in to the nucleus in the Sox2+ cells.
Moreover, analysis of
Wnt/JNK pathway activation through the immunodetection
of pJnk showed that
neither this signaling would be activated by Wnt in the sox2+
basal cells. These results
suggest that Wnt is regulating Sox2 expression in OE basal
cells although through
activation of another Wnt signaling pathway were B-catenin
and pJNK are not involved.