In vitro and in vivo endometrial growth inhibition by natural compounds evaluated as new therapeutic agents for endometriosis.

FERELLA L; BASTON JI; BILOTAS M; SINGLA JJ; OLIVARES C; MERESMAN G

San pablo

Congreso; 12th World Endometriosis Congress; 2014

World Endometriosis Society

Objective: To evaluate the effect of Wogonin, an active constituent of Chinese Herbal Medicine; two of the main antioxidant compounds found in rosemary extract: Carnosic Acid (CA) and Rosmarinic Acid (RA); and 1α,25-dihidroxivitaminD3 (VD3) in vitro and in vivo, in experimental endometriosis. These compounds demonstrated antiproliferative properties in several cancer models. Design: Primary cultures of human eutopic endometrial stromal cells from endometriosis patients and T-HESC stromal endometrial cell line were cultured with Wogonin, CA or RA for 24-h, or VD3 for 48-h. Endometriosis was surgically induced to BALB/c mice. Animals were assigned to different groups: RA-1mg/kg; RA-3mg/kg; CA-2mg/kg; CA-20mg/kg; Sham and Control. Materials and Methods: Cell proliferation was evaluated by MTS assay in primary and T-HESC cell cultures. The compounds were administered to animals daily intraperitoneally, started in post-operative day 14 and continued during 15 days. After treatment, animals were sacrificed, the abdomen was opened, lesions were counted and measured using the formula V=(4/3)r2R. Results: Wogonin 40, 80 and 160 µM, CA 10, 12.5 and 25 µg/ml and RA 25, 50 and 100 µg/ml significantly inhibited cell proliferation in T-HESC cell line (p