INVESTIGADORES
TRINKS Julieta
congresos y reuniones científicas
Título:
GB virus C (GBV-C) genomic RNA is present in specific lymphocytic cells in vivo.
Autor/es:
RUIZ V; GIORDANO M; MATHET V; RIVERO C; MINASSIAN M; CUESTAS M; FLEMING X; TRINKS J; OUBIÑA JR
Lugar:
Pinamar.
Reunión:
Congreso; XLI Reunión anual de SAIB, 10th Congress of the Panamerican Association for Biochemistry and Molecular Biology.; 2005
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular - Panamerican Association for Biochemistry and Molecular Biology.
Resumen:
INTRODUCTION: GBV-C -also
known as Hepatitis G virus- is a human virus whose replication cell target has
not been identified. OBJECTIVE: To investigate GBV-C cell tropism in peripheral
blood mononuclear cells (PBMC) from a viremic blood donor. MATERIAL AND
METHODS: Mononuclear and polymorphonuclear cells were obtained by standard
procedures using Ficoll/Triyosom centrifugation and Dextran sedimentation.
Monocytes were isolated by adherence to plastic and lymphoid subsets were
purified using specific monoclonal antibodies and magnetic beads. RNA was
extracted from cellular pellets and 1 mg was
seeded for RT-Nested PCR for both genomic [+] and anti-genomic [-] strands by
using primers specific for the 5 NCR of the virus. Amplicons were analyzed by
agarosa gel electrophoresis and Southern hybridization with a 32P-labeled
internal oligoprobe. RESULTS: GBV-C genomic RNA was consistently detected in B
cells but not in monocytes, CD4 [+] T cells, polymorphonuclear cells, nor in
the last wash from any cell population. CD8 [+] T cells proved positive in 2 of
4 assays. The anti-genomic GBV-C RNA (replicative form) was undetectable in all
cell populations by using the highly strand-specific enzyme Tth DNA polymerase.
CONCLUSIONS: GBV-C viral RNA is present in B and probably in CD8 [+] T cells in
vivo, as shown for the first time in a natural infection.