IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ADIPOKINES AND OXIDATIVE STRESS IN PERIVASCULAR ADIPOSE TISSUE: A LINK BETWEEN DIABETES AND CARDIOVASCULAR DISEASE.
Autor/es:
ISABEL M. QUESADA; JIMENA CEJAS; BEATRIZ CANNIZO; ANALÍA REDONDO; CLAUDIA CASTRO
Lugar:
Mendoza
Reunión:
Congreso; XXXI Reunión Anual de la Sociedad de Biología de Cuyo; 2013
Resumen:
Perivascular adipose
tissue (PVAT) has been shown to secrete a wide variety of adipokines and produces
reactive oxygen species via NADPH oxidase system. In type 2 diabetes (T2DM) PVAT-derived
cytokines may influence key steps of atherogenesis. Thus, we generate two
experimental models of T2DM. We fed wild type
(WT) mice and apolipoprotein E-deficient mice (ApoEKO) with fructose overload
(FF, 10%v/v) for 8 weeks mimicking a classical T2DM model (FF-WT) and cardiovascular
complications in T2DM mice model (FF-ApoEKO). Next we analyzed plasma
biochemical variables, gene expression profile of NADPH oxidase complex
subunits (Nox1, Nox2, Nox4, p22phox and p47phox) and adipokines (visfatin, MCP-1 and
MMP-9) in the PVAT of these two models. Biochemical variables show that
glycaemia and cholesterol significantly increased in FF-WT mice compared with
WT mice, while in FF-ApoEKO mice only glycaemia increased significantly versus ApoEKO
control mice. In WT mice, PVAT expression of Nox1, Nox4 and p47phox
genes increased significantly in the FF group. This increase was higher in FF-ApoEKO
mice. In regard to markers of inflammation only MCP-1 increased significantly
in FF-WT mice compared with WT. However, Visfatin, MCP-1 and MMP-9 were
markedly upregulated in FF-ApoEKO mice. Our results establish a relationship
between increased PVAT oxidative stress and adipokines and cardiovascular
complication associated with T2DM