INVESTIGADORES
SOSA ESCUDERO Miguel Angel
congresos y reuniones científicas
Título:
Quinolinic acid induces changes in expresión of coat proteins in areas of rat brain.
Autor/es:
BORGONOVO J; SOSA MA; SELTZER A
Lugar:
Pinamar
Reunión:
Congreso; XX Reunión Annual de la Sociedad Argentina de Neuroquimica.; 2005
Resumen:
Clathrin- mediated endocytosis (CME) is the best-understood
mechanism of macromolecule internalization. In brain, CME is
required for recycling of membrane proteins after the release of
neurotransmitters in the synapse. Clathrin coated vesicle (CCV)
formation is triggered by recruitment of assembly proteins as
adaptors AP-2 or AP180 onto membrane domains where different
receptors are concentrated. Association of GABA (A) receptors
and AP2 represents an important mechanism in the postsynaptic
modulation of inhibitory synaptic transmission. Striatal quinolinic
acid (QA) injections induce loss of GABAergic medium spiny
projection neurons, mimicking Huntington disease. We measured
expression of coat proteins in areas of rat brain .AP-2 (alpha 1 and
2 subunits) and AP-180 expression were studied by WB analysis
by using specific monoclonal antibodies. We observed that QA
induce a decrease of both subunits of AP-2 in striatum at 48 hr,
whereas the proteins significantly increase in the cerebellum. Alpha
1 subunit was increased in hippocampus and no changes were
observed in cortex or brainstem . AP180 expression was not
modified. We concluded that QA alters selectively the transport
via CCV in striatum and cerebellum, two main areas involved in the
control of movement.