INVESTIGADORES
SOSA ESCUDERO Miguel Angel
congresos y reuniones científicas
Título:
The in vivo trypanocidal effect of the diterpene 5-epi-icetexone.
Autor/es:
LOZANO E; STRAUSS M; RIVAROLA W; NIETO M; TONN C; VEGA I; SOSA MA
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica (SAIB).; 2012
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica
Resumen:
MI-P56.
THE TRYPANOCIDAL EFFECT OF THE
DITERPENE5-EPI-ICETEXONE
IN VIVO
Lozano E, Strauss M, RivarolaW, Nieto M, Tonn C,Vega I, Sosa M.
Instituto de Histología y Embriología, Mendoza. E-mail:
estebanlozano@fcm.uncu.edu.ar
T. cruzi
T. cruzi
is the causative agent of Chagas´ disease. When cultured,
this parasite is found mainly as epimastigote and, at lesser extent, as
trypomastigote. Recently, we have found that 5-epi-icetexone
(ICTX), obtained from a native plant, affects the growth of
epimastigotes and amastigotes. In this study,we evaluated the effect
of this compound on parasitic forms in Albino Swiss infected. The
distribution of parasites in the tissues and parasitaemia were
evaluated at different times after the infection. Parasitemia was
measured by counting of parasites in blood. Thirty five days after
infection, when parasitaemia declined, the animals were sacrificed
and some organs were removed, fixed and processed for
microscopy. Thin sections from each tissue were stained with
hematoxylin?eosin and observed with light microscope.We found
that parasitemia decreased when 0.3 mg of ICTX is administered.
This effect was lower than that of Benznidazole, although ICTX was
administered at lower doses and by a different via.We also observed
some nests of parasites in the heart of either controls or treated with
DMSO alone, while they were not observed in the mice treated with
ICTX. In addition, inflammatory infiltrates caused by parasites
were not observed in the heart of treated animals.We conclude that
this compound is effective against even if they reside in a
host.