INVESTIGADORES
SOSA ESCUDERO Miguel Angel
congresos y reuniones científicas
Título:
Prosaposin is detected in bull spermatozoa and it changes along epididymal transit
Autor/es:
AGUILERA AC; CARVELLI L; BOSCHIN V; SOSA MA
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica (SAIB); 2012
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica
Resumen:
Mammalian epididymis participates in sperm maturation through
its endocytic and secretory activity. Some secreted proteins are
known to be targeted to sperm membrane. Prosaposin (PSAP) is a
lysosomal protein, which is found in mammalian epididymal fluid
and spermatozoa. Here, we intended to study the possible presence
of PSAPin the plasma membrane of epidydimal spermatozoa from
bull (Aberdeen Angus). The epididymides were dissected into
caput, corpus and cauda and the sperm were obtained by slicing of
the tissue and centrifugation. Using specific antibodies (by IFI and
flow cytometry) we detected PSAP in the sperm of bulls and the
signal was stronger in the gametes of the corpus. This protein was
partially released by 0.3 M ClNa, indicating a noncovalent
association with the gamete. All these results were confirmed by
Western blot. In addition, we have observed that the protein is
mainly located in the equatorial area of the head and also in the tail
of the sperm. PSAP was also detected in luminal vesicles and
mostly in caput and corpus, suggesting that the vesicles could be the
vehicle of PSAP for acquisition by the spermatozoa. Intriguingly,
most of the changes occurs in corpus, indicating that this region
could be crucial for sperm maturation. These observations could
provide new insights into molecular rearrangement and protein
acquisition during epididymal maturation.