INVESTIGADORES
LOCATELLI Fernando Federico
congresos y reuniones científicas
Título:
Measuring crab´s memory: improving conditioning
Autor/es:
ANDINA MATIAS; KLAPPEBACH MARTIN; LOCATELLI FERNANDO; PEDREIRA ME; ROMANO A; FELD M
Lugar:
Huerta Grande
Reunión:
Congreso; Congreso de la SAN 2013; 2013
Institución organizadora:
Sociedad Argentina de Investigaciones en Neurociencias
Resumen:
Measuring crab´s memory: improving conditioning
Matías Andina; Martín
Klappenbach; Fernando F. Locatelli; Eugenia Pedreira; Arturo Romano and Mariana
Feld1
Laboratorio de Neurobiología de la
Memoria, Dto. Fisiología, Biología Molecular y Celular, Fac. Cs. Exactas y
Naturales, UBA ? IFIByNE, CONICET
1 mfeld@fbmc.fcen.uba.ar
Context-Signal
Memory (CSM) of the crab Neohelice
granulata has been exhaustively studied as a learning and memory model that
allowed to unravel details from processes such as consolidation,
reconsolidation, extinction and retrieval. However, a limiting feature of the
experimental design has been the high number of animals needed to draw
conclusions.
Using
the traditional device, it was demonstrated that paired presentations of the
context (CS) with the visual danger stimulus, VDS (US) increases the predictive
value of the CS thus eliciting an anticipatory response. This new paradigm was
named Contextual Pavlovian Conditioning (CPC) (Fustiñana et al., 2012).
Recently, we developed a new device
to measure the crab´s escape response in order to reduce the number of
individuals. For this purpose, the crab
is hold from the dorsal carapace over a cylinder that rolls as the animal tries
to move. A computer records distance and time, and controls stimulation.
Our challenge was to adapt CPC to
the new device. Here, we present data showing that learning and memory
retention can be clearly revealed in this new device using four times less
animals than in the traditional one. Moreover, it allows more detailed
measurements and a thorough description of the behavior.
This tool promises a whole new set
of possibilities to venture into learning and memory mechanisms and, applying
experimental approaches suited for reduced number of animals.