INVESTIGADORES
VENTURINO Andres
congresos y reuniones científicas
Título:
Oxidative metabolism of polyamines and embryonic development in Rhinella arenarum embryos exposed to azinphos methyl
Autor/es:
LASCANO C; DEL BRÍO D; FERRARI A; VENTURINO A
Lugar:
Puerto Varas
Reunión:
Congreso; XII PanAmerican Society of Biochemistry and Molecular Biology ?PABMB? Congress; 2013
Institución organizadora:
PABMB
Resumen:
Polyamines
(PA) are aliphatic polycations essential for the normal progression of
embryonic development. We have shown that the pesticide azinphos methyl (AM)
alters PA metabolism, resulting in an increase in putrescine and a decrease
in spermidine and spermine. The oxidative degradation of PAs generates
reactive species. Our objectives are to characterize the oxidative
degradation of PAs and to determine if there is a link between this process
and the alteration of embryonic development. Embryos of the toad R. arenarum were
employed and the activities of diamine oxidase (DAO) and acetylpolyamine
oxidase (APAO) were determined. Also, documentation of morphological
alterations and a correlation analysis between enzyme activities and
malformations were carried out. We observed a significant increase in DAO
activity in embryos exposed to 2 mg/L AM at the end of embryonic development
and a significant decrease in APAO activity at intermediate stages in embryos
exposed to 0,5-, 2- and 9 mg/L AM. The number of malformed individuals
increased in embryos exposed to 9 mg/L AM. However, no correlation between
DAO, APAO and malformations was observed. More studies are needed to
characterize the oxidative metabolism of polyamines and to determine which of
the degrading enzymes, if any, contributes to the alteration of R. arenarum embryonic
development. This work was supported by grants from: Univ. Nac. Comahue 04 I
004; ANPCyT PICT 214/07 and CONICET PIP 2010-2012 00655.