INVESTIGADORES
RODRIGUEZ VIRASORO Ramiro Esteban
congresos y reuniones científicas
Título:
Enhanced oxidative stress tolerance of tobacco transgenic plants overexpressing both ferredoxin-NADP(H) reductase and flavodoxin
Autor/es:
ANABELLA LODEYRO; RAMIRO ESTEBAN RODRIGUEZ VIRASORO; HUGO POLI; VANESA TOGNETTI; ESTELA VALLE; NESTOR CARRILLO
Lugar:
Bariloche, Argentina
Reunión:
Congreso; XXXIX Reunión Anual Nacional de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB); 2003
Resumen:
Ferredoxin-NADP(H)
reductases (FNR) catalyse the reversible electron transfer between two
molecules of obligatory one-electron carriers as ferredoxin and flavodoxin (Fd)
and a single molecule of NADP(H). Tobacco plants expressing Anabaena PCC7119 flavodoxin displayed
enhanced tolerance to a variety of oxidative stress conditions. Recently, plants
overexpressing FNR in chloroplasts were also obtained. In this work, we showed that
this tobacco plants overexpressing FNR were more tolerant to excess light or to
the reactive oxygen species generated by the herbicide methyl viologen (MV). We
also evaluated the tolerance to MV of transgenic plants overexpressing both FNR
and the cyanobacterial flavodoxin. These plants were obtained either by cross-fertilization
or by transient expression of FNR directed to chloroplasts or cytosol developed
by leaf infiltration with Agrobacterium
tumefasciens carrying the appropriate plasmid construct. Results related to
the enhanced tolerance to MV of these double transgenic plants will be
discussed.