Enhanced anti-proliferative effect in melanoma cells by catalase-magnetite nanocomplex

MUNICOY, SOFÍA; IBAÑEZ, IRENE L.; DURÁN, HEBE; BELLINO, MARTÍN G.

Buenos Aires

Congreso; VIII Meeting of the South American Group of the Society for Free Radical Biology and Medicine (SFRBM); 2013

South American Group of the Society for Free Radical Biology and Medicine (SFRBM)

Catalase, an enzyme degrading hydrogen peroxide, has been proposed as an anti-proliferative agent for tumor cells. The development of versatile catalase-magnetic nanocomplex remains challenging. Here we evaluate magnetite nanoparticles (MNPs) functionalized with catalase (MNP-CAT) in tumor cell proliferation. We synthesized MNPs by co-precipitation and mixed them with catalase; and human melanoma cells were incubated with CAT, MNPs or MNP-CAT. The MNPs stability was analyzed by Dynamic Light Scattering, catalase activity was determined spectrophotometrically and cell proliferation was evaluated by MTT assay. MNP-CAT exhibited high stability along time as compared with MNPs and catalase activity was well-preserved in MNP-CAT up to 42°C and in range of pH 3-9. Tumor cell proliferation decreased in presence of MNP-CAT as compared with CAT treatment while tumor cell proliferation was not affected by MNPs. Cells incubated with MNPs and exposed to magnets in order to remove MNPs reached similar levels of proliferation as compared with non-treated cells. We conclude that MNPs are promising carriers for catalase delivery improving the anti-proliferative effect in tumor cells with the advantage that MNP-CAT can be easily delivered or removed with magnetic guidance.