PERSONAL DE APOYO
MARQUEZ sebastian
congresos y reuniones científicas
Título:
StarD7 siRNA modulates ABCG2 expression, cell migration and proliferation in epitelial cell lines
Autor/es:
JESICA FLORES- MARTÍN; VIVIANA RENA; MÁRQUEZ S; GRACIELA PANZETTA -DUTARI; SUSANA GENTI DE RAIMONDI
Lugar:
Mendoza
Reunión:
Encuentro; 48 Annual Meeting XLVIII Reunión Anual th Argentine Society for Biochemistry and Molecular Biology; 2012
Institución organizadora:
SAIB
Resumen:
StAR-related lipid transfer domain containing 7 (StarD7) belongs to
the family of START proteins ubiquitously expressed, which are
implicated in lipid transport, metabolism, and signalling. Previous
results indicate that StarD7 silencing decreases ABCG2 multidrug
transporter level, cell migration, proliferation, and reduction in
phospholipid synthesis; whereas an increase in biochemical and
morphological differentiation marker expression was detected in the
epithelial choriocarcinoma JEG-3 cells. The present study was
undertaken to extend these data to other epithelial tumour cells to
establish the StarD7 function. In order to do this, we performed
StarD7 knockdown in hepatoma HepG2 and colon adenocarcinoma
HT-29 cells, two cell lines in which we previously demonstrated
higher StarD7 expression levels. StarD7 silencing, confirmed by
qRT-PCR and western blot, led to a decrease in the xenobiotic/ lipid
transporter ABCG2 at both the mRNA and protein levels. Also a
concomitant reduction in bromodeoxyuridine uptake was detected.
Wound healing and transwell assays revealed that HepG2 and HT-
29 cell migration was significantly diminished. Altogether these
findings provide evidence for a role of StarD7 in cell physiology
suggesting that it has a conserved function. Current work is in
progress to establish the mechanisms involved in these findings