INVESTIGADORES
CANIFFI Carolina Cecilia
congresos y reuniones científicas
Título:
Cardiovascular nitric oxide system activation via C-type natriuretic peptide: receptors and signaling pathways involved.
Autor/es:
COSTA MARÍA DE LOS ÁNGELES; ELESGARAY ROSANA; CANIFFI CAROLINA; FINELLA SEBASTIÁN; VISINTINI JAIME MARIA FLORENCIA; BALASZCZUK ANA MARÍA; ARRANZ CRISTINA
Lugar:
Milán
Reunión:
Congreso; Seventeenth European Meeting on Hypertension.; 2007
Institución organizadora:
European Society of Hypertension.
Resumen:
<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} .MsoPapDefault {mso-style-type:export-only; margin-bottom:10.0pt; line-height:115%;} @page WordSection1 {size:612.0pt 792.0pt; margin:72.0pt 72.0pt 72.0pt 72.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.WordSection1 {page:WordSection1;} --> C-TYPE NATRIURETIC PEPTIDE: RECEPTORS AND SIGNALING PATHWAYS INVOLVED                                         M.A. Costa (1), R. Elesgaray (1), C. Caniffi (1), S. Finella (1), F. Visintini (1), A. Burger (1), A. Fellet (1),                         A.M. Balaszczuk (1), C.T. Arranz (1) (1) FFyB. University of Buenos Aires, IQUIMEFA - CONICET. Fund. Barcelo-Argentina In previous studies we demonstrated that the diuretic and hypotensive effects of the atrial natriuretic peptid (ANP) involved the activation of nitric oxide synthase (NOS). Another member of the natriuretic peptide family is C-type natriuretic peptide (CNP) that is expressed in nervous system, vascular endothelium and heart, among others. CNP is less potent inducer of diuretic and natriuresis than ANP but is more potent in eliciting smooth muscle relaxation. Objective: The aim was to investigate changes in the mean arterial pressure (MAP, mmHg) and NO system induced by CNP and the receptor type and the possible mechanisms of signaling involved in this effects. Desing and methods: Nitrites and nitrates excretion (NOx, nmol/min.100g). NOS cathalytic activity (pmol/min.g tissue) was determined by measuring the convertion of L-[U14C]-Arginine to L-[U14C]-citrulline, after the addition of CNP (1ìM), cANP (4-23) (a selective NPR-C ligand, 1 ìM), Pertussis Toxin (PTx, Gi protein inhibitor, 800 ng/ml) in aorta artery, cardiac atria and ventricle from male Wistar rats. Results: MAP was diminished by CNP infusion (81±3 vs 95 ±4, p