“Responses to salinity in Cyrtograpsus angulatus: osmoregulation and energy stores”

ASARO, A.; DEL VALLE, J.C; LÓPEZ MAÑANES, A,A.

Congreso; XIII Congress- XXXI Annual Meeting Rosario Biology Society, Rosario; 2011

Studies on osmoregulatory flexibility and associated metabolic adjustments in various ecological relevant euryhaline crabs are lacking. The aim was to determine osmolality (O) and glucose levels (Glu) in hemolymph (HL) and glycogen content (G) in anterior (AG) and posterior gills (PG), chela muscle (M) and hepatopancreas (H) in male individuals (n=6-8) of C. angulatus from Mar Chiquita coastal lagoon exposed for 10 days to 40, 35, 10 and 6 ‰ salinity (S). O (mOsm x kg-1) and Glu (mg glu x ml HL- 1) were determined by cryoscopic osmometer and with Kit (Wiener Lab AA), respectively. G (mg x g tissue-1) was assayed in homogenates from AG, PG, M(sucrose 0.25M/EGTA-Tris 0.5mM, pH7.4) and H (Tris-HCl 0.1M, pH 7.4) by hydrolysis with aamyloglucosidase (0,2ml/ml). ANOVA was used for statistical analysis (p0.05). The results show that C. angulatus behaves as hypohyperegulator and suggests the occurrence of differential metabolic adjustment in relation to osmoregulatory status.