INVESTIGADORES
FERNANDEZ Natalia Jorgelina
congresos y reuniones científicas
Título:
Antimicrobial activity of essential oils against Paenibacillus larvae
Autor/es:
7. GENDE LB, AFONSO PIRES SM, FERNÁNDEZ N.J, DAMIANI N, CHURIO MS, FRITZ R, EGUARAS MJ
Reunión:
Simposio; 43rd International Symposium on Essential oil; 2012
Institución organizadora:
Faculdade de Cièncias de Lisboa
Resumen:
American
foulbrood is a serious bacterial disease that affects Apis mellifera colonies; the causative agent is Paenibacillus larvae [1]. The aim
of the study was to evaluate in vitro
the antimicrobial activity of 32 essential oils against P. larvae. Oils from 21 botanical species were analyzed by gas
chromatography (CG and CG/EM). All essential oils were classified according to the
composition of their main components in two groups: benzene ring compounds
(BRC) and terpene compounds (TC). Minimal inhibitory concentration (MIC) in MYT
broth [2] was assessed by the microdilution
method. Final serial dilution concentrations of the essential
oils ranged between 2,000-12.5 μg/mL. The bacterial isolates were collected from different Argentinas
region. The chromatographic analysis showed that a 67% of the
essential oils contained predominately terpene compounds, while the remaining
33% included mainly compounds with benzene rings. From the TC group, Cymbopogon citratus essential oil showed
the better antimicrobial activity against P.
larvae with MIC values between 150 and 250 mg/ml. The essential
oils from Aloysia polystachya and Mentha spp. had the lowest inhibitory activity. Among the oils from
the BRC group, one of the lowest MIC values was found with cinnamon essential
oil (Cinnamomun zeylanicum) being between 25 and 50 mg/ml; Origanum vulgare showed the highest
MIC values (350-400 mg/ml).
Essential oils, especially those with BRC in
their composition, presented inhibitory capacity against P. larvae
strains.