INVESTIGADORES
BLANCO Nicolas Ernesto
congresos y reuniones científicas
Título:
Tobacco plants expressing a cyanobacterial ferredoxin display increased oxidative stress tolerance
Autor/es:
CECCOLI, R.D.; BLANCO, N.E.; CARRILLO, N.
Lugar:
Mar del Plata
Reunión:
Congreso; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (S.A.I.B.); 2007
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Celular
Resumen:
Ferredoxins (Fd) are iron-sulfur proteins involved in many
different one-electron transfer pathways in plants, animals and
microorganisms. In chloroplasts and cyanobacteria, Fd distributes
the reducing equivalents generated in the photosynthetic electron
transport chain to various essential oxidoreductive pathways. Fd
levels decrease under adverse environmental conditions, affecting
severely plant physiology. Moreover, antisense Fd plants display
drastic alterations in growth and development. The aim of this study
is to evaluate the effect of the expression of a bacterial version of
this protein which is free from endogenous regulation on the
tolerance to oxidative stress conditions. We generated transgenic
tobacco plants expressing PCC7120 Fd in plastids under
the control of the cauliflower mosaic virus 35S promoter by
-mediated leaf disc transformation.
Immunoblots were used to confirm the expression of the alien Fd.
No phenotypic differences were observed between wild-type and
transgenic plants cultured in a growth chamber. We evaluated
oxidative stress tolerance using the redox-cycling herbicide
methyl-viologen (MV) that propagates reactive oxygen species.
The transgenic plants exhibit enhanced tolerance to oxidative
damage. This suggests that expression of the cyanobacterial Fd
might contribute to improve stress tolerance