Plasticity of mesenchymal stem cells (MSCs) from bone marrow (BM) of patients with lung and breast cancer (LCP and BCP)”.

HOFER, E. L.; FERNANDEZ VALLONE, V.; LAVOBSKY, V.; FELDMAN, L.; BORDENAVE, R. H.; VINCENT LA RUSSA; CHASSEING, N. A.

Austin, Texas, EE.UU.

Congreso; 5th International Conference on Mesenchymal and Non-Hematopoietic Stem Cells (MSCs).; 2009

International Society for Cell Therapy (ISCT).

PLASTICITY OF MESENCHYMAL STEM CELLS (MSCs) FROM BONE MARROW (BM) OF PATIENTS WITH LUNG AND BREAST CANCER (LCP and BCP). Hofer Erica L, Fernández Vallone Valeria B, Labovsky Vivian, Feldman Leonardo, Bordenave Raúl H, LaRussa Vincent, Chasseing Norma A. MSCs are able to self-renewal and differentiate. Previously, we observed a decrease of the cloning efficiency of MSC to give CFU-F, and of the CFU-F size in BM of untreated advanced BCP and LCP without bone metastasis vs healthy volunteers (HV). Proliferative activity of MSCs is directly proportional to their multipotentialy. To study the influence of tumor development on the relationship between self-renew and plasticity of MSCs, we evaluated the osteo-chondro-adipogenic differentiation of MSCs on 7BM-chemonaive-advanced-BCP, 7LCP and 7HV. BM-MSCs derived from low density 3rd passage were cultured in osteogenic, adipogenic or chondrogenic mediums.Adipogenic:100%HV-cultures responded achieving morphologies with characteristics of this linage vs 57%BCP and LCP. number of adipocytes/Petri-dish:HV=468+/-215a,b;LCP=98+/-66a;BCP=4+/-3b (a=p