RAT PROSTATE CYTOSOLIC XANTHINE OXIDASE MEDIATED METABOLISM OF ACETALDEHYDE TO ACETYL RADICALS

M.H. COSTANTINI; L.N. QUINTANS; M.E. MACIEL; J.A. CASTRO; G.D. CASTRO

Mar del Plata

Congreso; XLIII Reunión Anual de SAIB; 2007

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Repetitive alcohol drinking is known to lead to deleterious effects on prostate epithelial cells from humans and experimental animals. In previous studies from our laboratory we provided evidence about the presence in the rat ventral prostate of cytosolic and microsomal pathways of metabolism of ethanol to acetaldehyde and 1-hydroxyethyl radical and about the poor to null presence of alcohol dehydrogenase and aldehyde dehydrogenase. Acetaldehyde accumulation in prostate tissue and oxidative stress promotion were also observed. In the present study we report that in the ventral prostate cytosolic fraction, xanthine oxidoreductase is able to metabolize acetaldehyde to acetyl radical. The identification of the acetyl was performed by GC-MS of the silylated acetyl-PBN adduct. Comparison was made against reference adduct from acetyl generated in two chemical model systems. Acetyl was also detected using pure xanthine oxidase from butter milk. The generation of acetyl by the prostate cytosol was inhibited by allopurinol, oxypurinol, diphenyleneiodonium chloride, folic acid and ellagic acid at low concentrations. Previous and present results suggest that metabolism of ethanol to acetaldehyde and to 1-hydroxyethyl and acetyl radicals could be involved in the deleterious effects of alcohol drinking on prostate epithelial cells. Supported by CONICETand by a grant from UNSAM(PIB S06/89).