Overexpression and nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in acetaminophen acutely intoxicated rat

DELFANTE AE; RUIZ ML; DI CARLO MB; PERAZZO JC; MOTTINO AD; GHANEM CI

San Diego

Congreso; Experimental Biology 2012; 2012

American Society Pharmacology and Experimental Therapeutics division

The mechanism of acetaminophen (AAP) hepatotoxicity involves glutathione depletion, oxidative stress and mitochondrial dysfunction leading to necrosis. Recently, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was highlighted as a mediator of cell death. Aim: To evaluateGAPDH expression and subcellular distribution in liver of AAP acuteintoxicated rats. M&M: Male Wistar rats were injected i.p.with 1 g/kg b.w. of AAP (AAP group) or vehicle (C group) (n=4). After 24 h, liver GAPDH expression (mRNA and protein)was studied by RT-PCR and Western-Blot (WB). Nuclear, mitochondrial, and cytosolic GAPDH content was evaluated in hepatic subcellular fractions by WB. Results were expressed as mean±SD and statistically analized with Student-t-test (* p