Detection of Bean yellow mosaic virus, in soybean crops of Argentina

CAMPOS, EDUARDO; BEJERMAN, NICOLÁS; NOME, CLAUDIA; LAGUNA, GRACIELA; RODRIGUEZ PARDINA, PATRICIA

Congreso; IX World Soybean Research Conference; 2013

Argentina is one of the top soybean exporting countries in the world. As in other soybean producing regions, viral diseases constitute a phytosanitary problem. They may cause economic losses, both diminishing yields and affecting soybean exports, especially in the case of seed-borne viruses. Ten viruses have been identified infecting soybean crops in Argentina since the late 1970´s: Soybean mosaic virus (SMV), Alfalfa mosaic virus (AMV), Peanut mottle virus (PMV), Tobacco streak virus (TSV), Cucumber mosaic virus (CMV), Groundnut ringspot virus (GRSV)  and four begomoviruses: Soybean blistering mosaic virus, Tomato yellow spot virus, Bean golden mosaic virus and Euphorbia mosaic virus. During 2010/2011 growing season a survey was carry out, in different soybean producing areas of Argentina. A total of 277 symptomatic soybean samples were collected from different plots of Córdoba, Entre Ríos, La Pampa, Buenos Aires, Salta and Jujuy provinces. All samples were tested by ELISA using SMV, AMV, TSV, CMV, GRSV antiserums, and hybridization probes for begomoviruses. Some of the tested samples were negatives for allthe analyzed virus. One of these samples was mechanically inoculated to soybean and maintained under greenhouse for further analysis by transmission electron microscopy (TEM) , serology and RT-PCR. Flexuous filamentous particles were observed on dip preparations observed under TEM ,the samples gave a positive reaction when tested with anti-Potyvirus antiserum (AGDIA Inc) and PCR products of the expected size (1600pb) were obtained using PV2 degenerated primer (forward) and a oligo dT (reverse). The PCR product was cloned and sequenced. Sequence similarity comparisons demonstrated that this isolate has the highest nucleotide sequence identity (93%) with Bean yellow mosaic virus (GenBank: accession number AF185961.1). To our knowledge this is the first report of Bean yellow mosaic virus infecting soybean in Argentina.