CONICET | Buscador de Institutos y Recursos Humanos

Background: Inquilinus limosus are non-fermenting gram-negative bacilli of the alfa-Proteobacteria class. This species is increasingly isolated from cystic fibrosis (CF) patients. In general, and as expected to isolates from patients that undergo several antibiotic treatments, exhibit a multiple resistance pattern. However, the associated antibiotic resistance mechanisms are still unknown. Our goal is to elucidate putative genes related to microbial susceptibility or resistance in the genome of I. limosus. Methods: I.limosus MP07 with apparent high level of resistance to several antibiotics was isolated from a CF patient. The draft assembly genome sequence was determined by the 454 GS FLX pyrosequencing. Newbler assembler and on-line bioinformatics tools were used to analyze the sequence. Results: The whole genome of I. limosus was estimated to be 8.1 Mb; 84% is already sequenced with 97.3% assembled displaying an average GC content about 67.9% but reaching up to 73% in some regions. At the moment, 6,351 protein coding genes were identified. It was possible to assign a biological function to 68.7% (4,362) of the coding sequence, 30.4% (1,931) were found to be conserved hypothetical proteins. Eighty nine genes are likely involved in susceptibility or resistance to antibiotics and toxic compounds. Up to 19 could be coding for multi-drug resistance efflux pumps, while 21 for different classes of ß-lactam recognizing proteins as penicillin binding proteins. From them, four are homologous to ß-lactamases deposited in databases. No transposable elements or pathogenicity islands could be detected. Conclusion: We report the first draft assembly genome sequence of I. limosus which contains several genes that may be involved in the resistance to different antibiotic families and the potentially presence of various ß-lactamases that may explain the observed resistance pattern.

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