Effect of sildenafil on l-nitroarginine methyl ester (l-name) preeclampsia-like induced mouse model

MOTTA CE, GROSSO MC, ZANUZZI CN, PICCO NY, BELLINGERI RV, ALUSTIZA FE, VIVAS AB, ROMANINI MC, BARBEITO CG

Foz Iguazu

Congreso; V Latin American Symposium on Maternal Fetal Interaction & Placenta IV Latin American Symposium on Reproductive Immunology; 2013

Latin American Symposium on Maternal Fetal Interaction & Placenta and Reproductive Immunology

L-NAME decreases the vasodilator effect of nitric oxide and induces preeclampsiain mouse. Sildenafil inhibits the degradation of nitric oxideand increases vasodilation. To determine the effects of sildenafil onplacental development a L-NAME preeclampsia-like induced mouse modelwas used. Twenty pregnant mice were divided into 4 groups: 1) Control; 2)L-NAME; 3) sildenafil; 4) L-NAMEþsildenafil. L-NAME was administeredfrom day 7 of pregnancy and sildenafil from day 8 until day 16; animalswere sacrificed on day 17. Placental weights were recorded; placentalvascular endothelial were determined by lectin-histochemistry and lipidperoxide levels in placental homogenates were determined. Protocolswere approved by Ethic committee (UNRC). Data were analyzed byANOVA. Placental weight decreased significantly in both L-NAME and LNAMEþsildenafilgroups, also the percentage of mark in L-NAME group.However lipid peroxide levels showed no statistical differences. Treatmentwith L-NAME decreased weight and vascularization of placenta. Sildenafilmay counteract these changes in the placentas.