Analysis of bioactive fraction of Phoradendron liga (Gill ex H. & Am.) Eich. leaf extract

SELIS, A.N.; SGROI, N.A.; SGARIGLIA, M.A.; SOBERÓN, J.R.; VATTUONE, M.A.

Tafí del Valle, Tucumán

Congreso; XXVIII Annual Scientific Meeting of Tucuman Biology Society; 2011

Tucuman Biology Society

Lemon production of Tucumán could be sustainable and fungi-free if used natural products to combat them. The ethanolic extract of P. liga and their subextracts showed this capacity. These were obtained by extraction with increased polarity solvents (ethyl ether, dichloromethane and methanol), and were purified by column chomatography (CC) (Sephadex LH20, methanol as mobile phase); CC eluents were analized by TLC (Silica gel F254 plates, toluene / ethyl acetate / HCOOH / methanol 7:8:2:3 v/v/v/v as mobile phase) and the developed plates were revealed with: UV-vis, FeCl3, vainillin-sulfuric and NP/PEG reagents. The spots analysis suggests the presence of compounds such as naringin and neohesperidin (dihydrochalcone), mono and di-glycosilated flavonoids, a non-glycosylated flavonoid, phenolic compounds, chlorophyll, auron, and a genistein type isoflavonoid (with and without glycosilation). The isolated flavonoids are ascribed as responsible for antifungal activities of P. liga ethanolic extract.