ANTIFUNGAL ACTIVITY OF ZUCCAGNIA PUNCTATA: COMPARISON WITH COMMERCIAL FUNGICIDES

JIMENEZ, C.M.; SAMPIETRO, D. A.; SGARIGLIA, M.A.; SOBERON, J.R.; VATTUONE, M.A.

Tafi del Valle

Congreso; XXVIII Annual scientific meeting of the Tucuman biology association; 2011

Tucuman biology association

Argentina is an important cereal producer. Diseases restrict grain yields , especially those caused by Fusarium species. Commercial fungicides used for their control have limited efficiency and affect the environment. The aim of thi s work was to compare the fungicidal and fungistatic activity ofan ether fraction (EF) from the tincture of Z. punctata with commercial fungicides: pyraclostrobin (Py) and epoxiconazole (Ep). The dry material extracted by tincture of Z. punctata was partitioned with diethyl ether. The antifungal activity of the EF (5-250 ug de ME/ml) and the fungicides (0.5-50 µg/ml) were assayed by the microdilution method on F. thapsinum (Ft), F. verticillioides (Fv), F. graminearum sensu stricto (Fse), F. subglutinans (Fs) and F. bothii (Fb). Minimum fungicidal concentration (MFC), minimum inhibitory concentration (MIC), MFC/MIC, and inhibitory concentration 50 (IC50) were determined. IC50s indicated than EF (19-27 ug/ml) produced a similar inhibition in all strains. IC50s of Py and Ep depended on the fungus tested, the most sensitive being Fse (2.4 and 2 ug/rnl, respectively ). MFCs (100 ug/ml for Fv and Fs; 250 ug/ml for Fb and Ft and 50 ug/m l for Fse) of EF coincided with their MICs, indicating that the inhibition observed is due to fungicidal action.