INVESTIGADORES
PERIOLO Natalia
congresos y reuniones científicas
Título:
CD30 Antigen Expression Is Involved in Celiac Disease?.
Autor/es:
NATALIA PERIOLO, LAURA GUILLEN, MARCOS BARBOZA, SONIA NIVELONI, EDUARDO MAURIÑO, JULIO C BAI, ALEJANDRA C CHERÑAVSKY.
Lugar:
California, Estados Unidos.
Reunión:
Congreso; Digestive disease Week; 2008
Institución organizadora:
Digestive disease Week
Resumen:
CD30 antigen expression is involved in Celiac Disease. Background/aims: CD30 antigen is expressed after activation of normal B and T lymphoid cells. Different T cell activating stimuli induce CD30 expression on CD45RO precursors. The putative role of CD30 in celiac disease (Cel) is poorly known. Our aims are to determine CD30 expression after nonspecific activation of peripheral blood lymphocytes (PBL) and to examine CD30 expression in duodenal T cells from biopsies ex-vivo challenged with gliadin. Materials/methods: PBL from 10 healthy controls (Co) and 10 active adult Cel patients were incubated with 1 mg/ml of anti-CD3 for 5 days. Triple immunofluorescence (anti-CD45RO PE or -CD25 PE, -CD3 PerCP and -CD30 FITC)and flow cytometry analysis were performed at baseline and days 3 and 5 after incubation. CD30 antigen was immunohistochemically evaluated in paraffin sections of duodenal biopsies from 6 Cel patients and 6 Co. Triple immunofluorescence and flow cytometry analysis was performed on isolated intraepithelial and lamina propria lymphocytes (IEL and LPL). Biopsy specimens from 3 Co and 3 Cel were similarly evaluated after 3-hour incubation of biopsies with 100 mg/ml of crude gliadin.Results:
