Tissue microarray technology in the study of cellular antigen expression and Epstein Barr virus association of Hodgkin lymphoma

MAGALHAES, M.; GUIRETTI, D.; CHABAY, P.; MARIA VICTORIA PRECIADO; DE MATTEO, E.; ZALCBERG, I.; HASSAN, R.

Colonia, Alemania

Congreso; 7th International Symposium on Hodgkin Lymphoma; 2007

European Hematology Association

The tissue microarray technology (TMA) allows the simultaneous study of many tissues and it is increasingly applied in cancer research. Hodgkin lymphoma (HL) is characterized by a low number of neoplastic cells, raising the question if TMA methodology can warrant a sufficient tumor representation for pathology studies. The objective of this study was to validate TMA for studies of cellular antigen expression and Epstein-Barr virus (EBV) association in HL. A TMA was constructed using a tissue arrayer (Beecher Instruments) including 56 pediatric HL diagnostic tumors, with 2 representative cores (1 mm diameter) of each sample. The phenotype was evaluated by CD30 and CD15 immunostaining. EBV association was determined by EBER-ISH and expression of EBV latent membrane protein LMP1 and LMP2A by immunohistochemistry (IHC). CD30, LMP1 and EBER-ISH detection was also performed in conventional slides, showing expression in 89%, 48% and 37% of the cases, respectively. Concordance between conventional and TMA detection was 100%. Analysis of LMP2A was performed in 51 cases; 12 cases showed membrane or para-nuclear (dot) immunostaining. All LMP2A+ cases showed also LMP1 expression, while 10 were LMP2Anegative/ LMP1+. Core duplication allowed to improve the number of available cases for CD30 (from 51 to 55 cases, p