PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
initial conidia adhesión of Aspergillus niger and its role in pellets formation
Autor/es:
COLIN VERONICA; BAIGORI M.D; PERA, LICIA M
Lugar:
Rosario, Pcia de Santa fe
Reunión:
Congreso; SAIB 42 th Annual Meeting. XLII Reunión Anual; 2006
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Introduction: Environmental conditions markedly influence the
growth pattern of filamentous fungi, which can range from a
pelleted to a dispersed filamentous form, influencing in this way
both the growth rate and the product formation. Although pellets
formation is primarily due to conidia agglomeration, the involved
mechanism has not been clearly elucidated. The aims of this
work were to quantify the initial conidia adhesion of A. nigerA. niger
under different environmental conditions and to establish its role
in pellets formation. Methods: Assays of conidia adhesion were
done in 96-well polystyrene microtiter plates by using
sulforhodamine B staining to quantify the adherent conidia. The
adhesion unit was defined as the amount of adherent substance
that produces an increase in absorbance at 570 nm of 0.01 per
min per ml of culture. The effect of modifications in the
environmental conditions was tested by changing either the
temperature of incubation or the initial pH of the medium as well
as by the addition of either CaCl2 or FeCl3. Results and
conclusions: Differential adhesion degree in response to
environmental conditions was detected. Under our experimental
condition, initial adhesion units lower than 0.4 obtained either at
initial pH 2 or in presence of 1 g/l FeCl3 were not compatible with
pellets formation. [This work was supported by grants 693/04
CONICET and PICTO 761].2 or FeCl3. Results and
conclusions: Differential adhesion degree in response to
environmental conditions was detected. Under our experimental
condition, initial adhesion units lower than 0.4 obtained either at
initial pH 2 or in presence of 1 g/l FeCl3 were not compatible with
pellets formation. [This work was supported by grants 693/04
CONICET and PICTO 761].3 were not compatible with
pellets formation. [This work was supported by grants 693/04
CONICET and PICTO 761].