ROL DE LOS LIPIDOS Y LA TRANSCRIPCIÓN GÉNICA EN LA MADURACIÓN DEL OVOCITO INDUCIDA POR TESTOSTERONA

ARIAS TORRES AJ., P¨¢EZ JB., AJMAT MT., B¨¹HLER MI. Y ZELARAY¨¢N LI.

Tucum¨¢n

Jornada; XXIX Jornadas Cient¨ªficas Asiciaci¨®n de Biolog¨ªa de Tucum¨¢n; 2012

Asociaci¨®n de Biolog¨ªa de Tucum¨¢n

ROLE LIPIDS AND GENOMIC TRANSCRIPTION IN OOCYTE TESTOSTERONE INDUCED MATURATION Arias Torres AJ., P¨¢ez JB., Ajmat MT., B¨¹hler MI., Zelaray¨¢n LI.INSIBIO-UNT.Tucum¨¢n. anajoarias@hotmail.com                                    Oocyte maturation is a progesterone-mediate process that occurs in absence of genomic transcription. Although progesterone (P4) is a potent promoter of R.arenarum oocyte maturation in vitro, little is known about other steroids in this process. In this study we analyze the role of lipids and genomic transcription in the mechanism testosterone (T) action during oocyte maturation. Oocyte maturation was induced by adding T (10-6 M) and time--response curves were performed with actinomycin D (2mM), Quinacrine (0-20mM) (PLA2 inhibitor) and indometacin (0-50¦ÌM) (COX inhibitor) were used. In order to analyze participation of phospholipid hydrolysis during maturation we used neomycin (0-2 mM). P4 (10-6 M) was used as control. Meiosis resumption was scored by germinal vesicle breakdown (GVBD) at 22 h. Our results shown that in R.arenarum oocytes T is a steroid inductor as efficient as P4. Maturation is induced by T-independent genomic transcription (95¡À3 %GVBD). Inhibition of formation of AA by quinacrine does not affect oocyte T induced maturation. COX inactivation by indometacin had an inhibitor effect on oocytes and follicles maturation. T induced maturation was inhibed by neomycin in a dose dependent manner. In R. arenarum T induces maturation independent genomic transcription manner, which participate in the second messenger derived from membrane phospholipids.