INVESTIGADORES
MÜLLER Gabriela Leticia
congresos y reuniones científicas
Título:
GLYCINE RICH RNA BINDING PROTEINS IN TOMATO FRUITS: A NOVEL POSITIVE REGULATION OF EXPRESSION
Autor/es:
MÜLLER GABRIELA; TRIASSI AGUSTINA; MARÍA V. LARA; DRINCOVICH, MA. FABIANA; ANDREO CARLOS S.
Lugar:
MENDOZA
Reunión:
Congreso; XLVIII Reunión científica anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; 2012
Resumen:
In this study, the analysis of GRP1 gene family was undertaken in
Micro-Tom tomato fruit. Three genes LeGRP1 (LeGRP1a-c) were
identified which are highly homologous to each other and to GRPs
of related species. Three transcriptional products for each gene
were identified, the un-spliced pre-RNA, the mature mRNA and the
alternative spliced mRNA (preLeGRP1a-c , mLeGRP1a-c and asLeGRP1a-c). LeGRPs show the classical N-terminal end RNA
recognition motif and the C-terminal end glycine-rich region,
suggesting conservation of functional properties. The levels of all
transcriptional forms of and the total immunoreactive
LeGRP1 decrease during tomato fruit development from immature
green to red ripe. Particular circadian profiles of expression were
detected for each transcriptional form. During ripening
off the vine after mature green, the levels of all transcriptional
forms increased at 20ºC but not at 4ºC. The correlation
analysis of the expression of all transcriptional forms
suggests a novel model of positive regulation of expression in
tomato fruit, in which each immature form of
positively correlates with each other and the form
correlates depending on the stage. The results obtained reveal a
complex pattern of expression of GRP in tomato fruits suggesting
these proteins as key regulators of important processes in fruits.