PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Proteomic study of Pichia jadinii M9 revealed differential proteins expression under chromium stress
Autor/es:
VERÓNICA IRAZUSTA; CRISTINA ESTEVEZ; LUCÍA INÉS FIGUEROA DE CASTELLANOS
Lugar:
Mar del Plata
Reunión:
Congreso; SAMIGE Sociedad Argentina de Microbiología General; 2012
Resumen:
Organisms subjected
to metal exposures in their natural environments generally have had develop
resistance mechanisms. Pichia jadinii M9 (GenBank
accesion number FJ865435), yeast isolated from textile factory effluents in
Tucumán, Argentina, has the ability of supporting high amounts of chromium metal
by a slow down in its growth rate.
In order to understand the mechanism involved in Pichia jadinii M9 resistance to chromium it was conducted a proteomic approach. Analyzing
by mono-dimensional gel electrophoresis the crude cells extracts, it was
observed differential bands expressions between cells with or without chromium.
Further, with the aim of studying these differences, two-dimensional
electrophoresis analyses was carried out by means of an isoelectric focusing
(11-cm strips, 3-11NL pH) and a SDS-PAGE (12.5%) for the first and second
dimensions, respectively. Gels were coomassie-stained, scanned and analyzed
with Image Master Program. 2-D analysis of Pichia
jadinii M9 revealed that 18 h chromium exposure produced an over-expression of 17 proteins.
Identification of differentially expressed proteins was performed by MALDI
TOF/TOF. Eleven of 17 spots identified belonged to heat shock proteins. Elongation
factor 1 alpha, methionine synthase, peptidyl-prolyl cis-trans isomerase and
vacuolar ATPase VI complex A, were also found over-expressed at high chromium
concentrations. The results obtained in the present work show that when exposing Pichia
jadinii M9 to 0.5 mM chromium concentration,
it is produced a differential protein expressions, some of them directly
involved in cell resistance mechanism