Impact of high fructose diet on visceral adiposity functionality

ALZAMENDI A; GIOVAMBATTISTA A; RASCHIA A; MARRA C; REBOLLEDO OR; GAILLARD RC; SPINEDI E

San Francisco, USA

Congreso; 90th Endocrine Society Meeting; 2008

The Endocrine Society

The aim of the present work was to evaluate changes in lipid metabolism and endocrine function of the visceral adipose tissue (VAT) in male rats with metabolic syndrome (MS) induced by a high fructose diet (HFD) (10% in the drink water [F10]) during three weeks. For this purpose, normal Wistar (F0) and F10 rats were sacrificed in basal condition and trunk blood was collected. Immediately after, VAT pads were dissected to determine in vitro the composition and release (by HPLC) of non-esterified fatty acids (NEFA) and to isolate adipocytes. These cells were 30 min incubated, without and with insulin (0.1-10 nM), for further evaluation of leptin secreted into the medium. Our data indicate that F10 rats displayed significant (p