Effect of INGAP-PP upon islet glucokinase activity and glucose metabolism

MAIZTEGUI B ET AL; GAGLIARDINO JJ

Rio Grande do Norte

Jornada; ISG 2011. Annual Meeting of the EASD Islet Study Group; 2011

EASD

Background and Aims: INGAP-PP enhances insulin secretion and increases β-cell mass; however, its possible effect upon islet metabolism is still unknown. Thus, we have studied the effect of INGAP-PP upon insulin secretion, glucose (G) metabolism and glucokinase (GK) activity in cultured islets isolated from normal rats. Methods: Islets were isolated from pancreases of normal adult male Wistar rats by collagenase digestion and cultured for 4 days in RPMI 1640 pH 7.4, containing 2 g/L NaHCO3, 5% (v/v) fetal calf serum, 1% penicillin/streptomycin and 10 mM G in a humid atmosphere (5% CO2/95% O2), with or without the addition of 10 µg/ml INGAP-PP. The islets were thereafter rinsed in KRB and preincubated in 1.0 ml of KRB containing 1.5% (w/v) BSA and 3.3 mM G at 37 °C for 45 min. After this period, groups of islets were incubated with 3.3, 8.3 or 16.7 mM G to study insulin release, G metabolism (14CO2 and 3H2O production from labeled glucose) and GK activity (bioassay). Results: C vs. INGAP-PP (* p