SEARCH FOR MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS (MAP) ANTIGENS FOR THE DIAGNOSIS OF PARATUBERCULOSIS (PTB)

MARIA LAURA MON; MARIANA VIALE; BASCHETTI GUIDO; FIORELLA ALVARADO PINEDO; ANDREA GIOFFRÉ; GABRIEL TRAVERÍA; PETER WILLEMSEN; DOUWE BAKKER; ROMANO, MI

Sydney

Congreso; 11th International Congress of Paratuberculosis; 2012

International Association for Paratuberculosis

Since the sequencing and analysis of the entire Mycobacterium avium subsp. paratuberculosis (MAP)genome was obtained, several specific proteins have been detected and their immunoreactivityevaluated. However, individual antigens have been able to identify only a subset of PTB-infectedanimals. Then, a mix of antigens could be a good candidate for serological diagnosis. Several studieshave attempted a high-throughput production of MAP recombinant proteins. The present study aimed toevaluate the antigenic capacity of 54 recombinant proteins of MAP to be recognized specifically by serafrom animals with PTB and then develop a cocktail of selected antigens to be evaluated by ELISA.