INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression pattern of xpax1, xpax9 and xunc genes and their participation in the sclerotome of the amphibian Xenopus laevis
Autor/es:
SANCHEZ R. S.; SANCHEZ S. S.
Lugar:
Tafi del Valle, Tucumán
Reunión:
Jornada; XXVIII Jornadas Científicas Asociación de Biología de Tucumán Tafí del Valle, Tucumán; 2011
Institución organizadora:
Asociacion de Biologia de Tucumán
Resumen:
The anurans body plan diverges
from that of other vertebrates. With respect to the skeleton, the
frogs are characterized by a truncated axial skeleton (AS) with absence
of discrete caudal vertebrae (CV). However, the ability to form elements of the CV is present in someanurans, suggesting that the molecular machinery that regulates their formation is still present in this group and the absence of these elements could be dueto alterations in the expression of aset of genes in the caudal region of the embryo.
The
AS develops from the sclerotome, a cell mass derived from the somites.
Those cells take up positions around the notochord and neural tube for
later differentiation to cartilage and bone of the spine. Studies in fish, birds and mammals showed that pax1, pax9 and uncx play a centralrole in the development of AS promoting the proliferation of sclerotomeand the formation of cartilaginous condensations. Loss of function of these genes results in severe abnormalities in the spine with absence of vertebral elements. In this work we characterize the expression pattern of the Xpax1, Xpax9 and Xunc genes in Xenopus by in situ
hybridization and RT-PCR. We found that these factors are expressed in
the sclerotome. The gain of function approaches by overexpressing the pax genes suggest their potential role in the development of the X. laevis spine.