“Study of biochemical-physiological parameters in the euryhaline crab Neohelice granulata from Mar Chiquita coastal lagoon (Bs.As.) exposed to zinc”

PANELO, L.C.; PANZERI,A.; LÓPEZ MAÑANES, A.A.; DEL VALLE, J.C

Encuentro; XXVII Annual Scientific Meeting Tucumán Biology Association; 2010

In Mar Chiquita coastal lagoon zinc (Zn) reaches levels up to 1224ugL-1. We studied the effect of Zn exposure of N. granulata upon different osmoregulatory status on key biochemical-physiological parameters: Total, (T), levamisole-insensitive:(LI) and levamisole-sensitive:LS alkaline phosphatase (AP) activities in chela muscle and hemolymphatic glucose (HG). Adult male crabs acclimated for 10 days upon hyper or hyporegulatory conditions were exposed for 96h without (control) or with Zn (Zn- exposed) (1224ugL-1). AP activities (nmolpNP x min-1x mgprotein-1) were assayed in muscle homogenates (0.25 M sucrose/EGTA-Tris 0.5 M pH 7.7) by hydrolysis of pNPP (9.5mM) in 4mM MgSO4/100 mM Tris/HCl (pH 7.7) with and without 16mM levamisole. HG (mg x ml-1) was assayed in supernatant (10000xg) (Kit GlicemiaAA Wiener-lab). Upon hypo or hyperregulation, T, LI and LS AP activities were similar in the chela muscle of Zn exposed and control crabs (ANOVA, p>0.05). Upon hyporregulation, no differences were found in GH (ANCOVA p>0.05). Upon hyperregulation, a higher GH (100%) was found in Zn-exposed crabs, although values were no significantly different (p