Bioguided isolation of an ellagic derivative from Caesalpinia paragueriensis bark infusion

SGARIGLIA, M. A.; SOBERÓN, J.R.; SAMPIETRO D.A.; QUIROGA, E.N.; VATTUONE, M. A.

Tafí del Valle

Congreso; XXVI Annual Scientific Meeting of Tucuman Biology Association; 2009

Tucuman Biology Association

Hyaluronidases (HYAL) are enzymes that depolymerize hyaluronic acid of extracelular matrix of mammalian tissues during inflammatory processes. Infusions and decoctions from Caesalpinia paraguariensis bark, and Cestrum parqui leaves and flowers are popularly used because their antiinflamatory properties. We investigated the HYAL inhibitory activity of extracts from the species described above, and determined the chemical nature of compounds present in bioactive extracts. The HYAL inhibitory activity was assayed by Reissig method, and was expressed as inhibition percent (%I); aspirine, gallic acid and (+)-catechin were used as controls. C. paraguariensis infusion was successively extracted with ethyl ether, chloroform and methanol. Methanolic fraction (MF) was bioactive and was then fractionated through Sephadex LH-20/Methanol. Bioactive sub-fractions were analyzed by two-dimesional TLC and UV-Vis spectroscopy. The content of tannins and anthocyanidins of biactive fractions were determined. The %I for Infusion, MF and MF without tannins were: 99,97±0,03 (50 ppm), 73,90±2,3, 71,30±1,30, respectively. The inhibitory activity was partially attributable to anthocyanidins (30,30±0,55; pelargonidin and delphinidine) and flavones and isoflavones (51,30±2,00). At present the isolated compounds are being identified by MS and NMR.