INVESTIGADORES
BONETTO Fabian Jose
congresos y reuniones científicas
Título:
FPGA implementation of a "Dynamic-Clamp"
Autor/es:
BAST, WALTER; DELLAVALE, DAMIAN; BONETTO, FABIAN
Reunión:
Conferencia; SPL 2011; 2011
Resumen:
The dynamic-clamp electrophysiological technique allows
the mimicking of the electrical effects of ion channels
embedded into the membrane of an intracellularly recorded
cell. Dynamic-clamp relies on the establishing of a loop
between the injected current and the recorded membrane
potential. In this work, a real-time dynamic-clamp system
was implemented on a Field Programmable Gate Array
(FPGA). The system proposed allows the concurrent
controlling of the activation of several ion channels. The
architecture of the instrument developed is based on two
main modules: a state machine (as the logic arbiter) with
the ADC and DAC interface, and a signal processing
module. The state machine controls the converters along
with the serial data-transfer interface (SPI). The signal
processing module implements the algorithms needed to
mimic several HH-type ion channel conductances.
Extensive simulations of the device operation were made,
showing that the instrument developed implements
effectively the dynamic-clamp control loop, with a
maximum operation frequency of 532 kHZ. The main
application of this instrument is the manipulation of ion
channels.