EFFECT OF AN I.C.V. INJECTION OF AGGREGATED BETA-AMYLOID (1-42) ON -DEGRADING ENZYMES IN THE RAT PREFRONTAL CORTEX

CANGIANO B; CORTEZ M; SANCHEZ M; ALBA R; ANZULOVICH AC; DELGADO SM; NAVIGATORE FONZO LS

MENDOZA

Congreso; SBCuyo 2022; 2022

among the elderly. The accumulation of amyloid- (A ) peptides in the brain of Alzheimer s disease patients is associatedwith cognitive deficit, loss of memory, and alterations in the circadian rhythms. Enzymes with A -degrading activityinclude members of the zinc metalloendopeptidase family. Among them, the ones with the most physiological relevance in the brain are endothelin-converting enzyme (ECE) and insulin-degrading enzyme (IDE). Brain-derived neurotrophicfactor (BDNF) and its receptor (TrkB) play an important role in the synaptic plasticity underlying memory and learning.Previously, we observed A -injected rats. The objective of this work was to investigate the effects of an i.c.v. injection of aggregated beta-amyloid(1-42) on daily patterns of ECE and IDE expression, as well as on oscillating BDNF and TrkB mRNA levels, throughouta 24 h period, in the rat prefrontal cortex. Four-month-old male Holtzman rats were divided into two groups defined as-injected ( for 24 h. IDE, ECE, BDNF, and TrkBmRNA levels were determined by RT-PCR. -degrading enzymes and cognition-relatedfactors varies on a daily basis in the prefrontal cortex andrhythms. -degrading enzymes andconsequently could affect the transcriptional activity of the endogenous cellular clock.