CONICET | Buscador de Institutos y Recursos Humanos

BACKGROUNDHSP20s are 20 kDa heat shock proteins involved in preventing protein aggregation during heat and drought stress responses in numerous plant species. They are conserved across all living organisms, yet their study in Cannabis sativa var. hemp (Cs) has been limited. Cs is one of the earliest domesticated crops, finding applications in various industries such as fiber, food, and oil production. It holds particular significance in medical contexts, especially for pain treatment and addressing refractory epilepsy stemming from neurological disorders. While genotyping and phenotyping of Argentine varieties have only recently commenced, the characterization of CsHSP20 in particular remains ambiguous.RESULTSThis study involves the analysis and characterization of the CsHSP20 subfamily through diverse bioinformatics tools. These tools encompass sequence analysis, chromosomal mapping, gene structure examination, and the utilization of publicly accessible RNAseq data from the Cs genome. Forty members of the CsHSP20 subfamily were identified and annotated. Noteworthy is the discovery of eight CsHSP20s situated on chromosome 06, forming a cluster of tandemly arranged genes. Moreover, the scrutiny of public RNA-seq data unveiled six CsHSP20s displaying upregulation under conditions of drought-induced stress.CONCLUSIONSThis investigation represents the initial instance of characterizing the CsHSP20 subfamily in Cannabis sativa, shedding light on its involvement in stress responses. The findings underscore the transcriptional activity of these members and the presence of tandemly arranged genes within the Cs genome. These results align with previously reported data in numerous other plant species such as Arabidopsis, tobacco, tomato, potato, and grape.

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