STARVATION INDUCES SMALL EXTRACELLULAR VESICLES RELEASE IN PANCREATIC CANCER CELLS WITH THE PROPRIETY OF AUTOPHAGY INDUCTION EFFECT

DANIEL GRASSO; MAXIMILIANO A. DIAZ; GIULIANA NARVÁEZ; DANIELA L. PAPADEMETRIO; ELIDA ALVAREZ; MARIA NOÉ GARCIA

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Sociedad Argentina de Investigación Clínica

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive disease with a survival rate to 5 years of less than 5%. Autophagy, a cellular degradative and recycling process, plays an important role in the hypoxic and low nutrient tumor microenvironment of PDAC cells. Extracellular vesicles (EVs) are tiny vesicular structures released by all cell types with implications in intercellular communication. The aim of this work was to describe the tumor derived EVs response to starvation. Firstly, we set up the quantitative immunodetection of EVs by means of image cytometry. Additionally, we developed the quantitative total EVs detection using a red fluorescent dye. Then, the PDAC cell line Panc-1 was submitted to starvation for 30 and 60 min. In the conditioned medium, we detected a significant increase of released EVs for both times (p